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<Original Article> Thyrotropin Regulates the Expression of mRNA for G-protein α-Subunits (αs and αi2) in FRTL-5 Cells
https://doi.org/10.34429/00003061
https://doi.org/10.34429/00003061471b09b4-02ee-4e36-baeb-1f62db96fab5
名前 / ファイル | ライセンス | アクション |
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AN10044054_11_03_04.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-05-18 | |||||
タイトル | ||||||
タイトル | <Original Article> Thyrotropin Regulates the Expression of mRNA for G-protein α-Subunits (αs and αi2) in FRTL-5 Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Thyrotropin | |||||
キーワード | ||||||
主題 | G-protein | |||||
キーワード | ||||||
主題 | αs | |||||
キーワード | ||||||
主題 | αi | |||||
キーワード | ||||||
主題 | FRTL-5 Cell | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.34429/00003061 | |||||
ID登録タイプ | JaLC | |||||
著者名 |
PENG, Xiao-ding
× PENG, Xiao-ding× HARAGUCHI, Kazutaka× ENDO, Toyoshi× ONAYA, Toshimasa |
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内容 | ||||||
内容記述タイプ | Other | |||||
内容記述 | In order to determine the molecular mechanism of regulation of the GTP-binding protein (G-protein)-adenylyl cyclase system by thyrotropin (TSH), we examined the expression of mRNA for G-protein stimulatory α subunit (αs) and inhibitory α subunit (αi2) by the reverse transcription-polymerase chain reaction (RT-PCR) method. TSH (10 mU/ml) stimulated the expression of αs mRNA in FRTL-5 cells by 40% after 6 hr, which was gradually reduced to a normallevel by 24 hr. Expression of β-actin mRNA was unchanged. In contrast, expression of αi2 mRNA was reduced by 25% after 6 hr and returned to the normal level by 24 hr. This concomitant change increased the relative abundance of αs to αi2. The effect of TSH on the expression of αs mRNA was mimicked by dibutyryl cAMP and forskolin. These findings show that the expression of G-protein subunits is regulated by TSH via a cAMP-dependent pathway on the mRNA level. | |||||
出版者 | ||||||
出版者 | 山梨医科大学医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0912-0025 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10044054 | |||||
書誌情報 |
山梨医科大学雑誌 en : 山梨医科大学雑誌 巻 11, 号 3, p. 53-58, 発行日 1996 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |