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<Original Article> Study on Lymphokine Activated Killer(LAK) Cells I. Improved LAK cell induction in vitro
https://doi.org/10.34429/00003229
https://doi.org/10.34429/00003229712859b0-0bac-4783-ae13-6ccff5b48e53
名前 / ファイル | ライセンス | アクション |
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AN10044054_03_03_03.pdf (650.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-05-18 | |||||
タイトル | ||||||
タイトル | <Original Article> Study on Lymphokine Activated Killer(LAK) Cells I. Improved LAK cell induction in vitro | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | LAK | |||||
キーワード | ||||||
主題 | IL-2 | |||||
キーワード | ||||||
主題 | Adoptive immunotherapy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.34429/00003229 | |||||
ID登録タイプ | JaLC | |||||
著者名 |
Iizuka, Hidehiko
× Iizuka, Hidehiko× Naganuma, Hirofumi× Yabusaki, Noboru× Komatsu, Hideki× Sakihama, Toshiko× Iimuro, Yuji× Yamamoto, Yasuyoshi× Fujii, Hideki× Yamamoto, Masayuki× Matsumoto, Yoshiroh× Sugahara, Katsuhiko× Nukui, Hideaki× Ueno, Akira× Nakajima, Yasuo× Tasaka, Kachio |
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内容 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The optimum incubation period, serum or plasma concentration, recombinant interleukin 2(rIL-2) concentration, cell density and kind of culture bottles for inducing lymphokine activated killer (LAK) cells in vitro from normal peripheral blood lymphocytes (PBL) were examined. Culture of PBL, at a high cell density of 2×10^6/ml with 9 U/ml of rIL-2(TGP-3) and 2% of serum (or plasma) for 3 days in a flat bottle, was most effective, although the cell density of 1×10^6/ml and 3 U/ml of rIL-2 has been used so far. ABO matched allogeneic frozen plasma was shown to be as effective to induce LAK cells as AB sera. This Inodification could reduce the volume of medium, number of culture bottles and CO2 incubator space, and cost required in mass culture for practical use. | |||||
出版者 | ||||||
出版者 | 山梨医科大学医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0912-0025 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10044054 | |||||
書誌情報 |
山梨医科大学雑誌 en : 山梨医科大学雑誌 巻 3, 号 3, p. 97-103, 発行日 1988 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |