{"created":"2023-05-18T10:11:05.272884+00:00","id":2795,"links":{},"metadata":{"_buckets":{"deposit":"3f2c7a2c-9501-4a2a-bd28-2eefa4d8253b"},"_deposit":{"created_by":5,"id":"2795","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"2795"},"status":"published"},"_oai":{"id":"oai:yamanashi.repo.nii.ac.jp:00002795","sets":["205:206:220"]},"author_link":["13122","13123","13124","13125","13126","13127","13128","13129"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"41","bibliographicPageStart":"31","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"山梨医科学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Yamanashi medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okamoto, Hirotaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yatomi, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Kaneo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamane, Tetsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitsumata, Masako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chen, Wen-Tien","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, Yukio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujii, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"内容","attribute_value_mlt":[{"subitem_description":"Objective: Sphingosine 1-phosphate (Sph-1-P), a bioactive lysophospholipid present in the plasma, is released from activated platelets. Our previous study demonstrated that Sph-1-P promoted the spreading on and migration of human umbilical vein endothelial cells (HUVEC) through the extracellular matrix (ECM), suggesting a possible induction of cell surface proteases in the Sph-1-P activated endothelial cells. In the present study, we examined whether seprase, a type II transmembrane serine protease (TTSP) usually absent in tissue cells, can be induced in endothelial cells activated by Sph-1-P. Methods: HUVEC migration through ECM was examined by modifi ed Boyden chamber assay. Western blotting and immunoprecipitation, using anti-seprase monoclonal antibodies (mAbs), were used to confi rm the seprase expression of HUVEC. Results: We show that Sph-1-P enhanced expression of active form seprase in a time- and dose-dependent manner in HUVEC. The Sph-1-P inducible active form seprase could be blocked by pertussis toxin and by C3 transferase, which inactivate Gi-type heterotrimetric G proteins and Rho, respectively. Conclusion: These results show that Sph-1-P can regulate migration of endothelial cells by inducing active form seprase expression, which, in turn, is mediated through a Gi-coupled cell surface receptor and the Rho protein.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34429/00002788","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"山梨大学医学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11839837","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-5091","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-27"}],"displaytype":"detail","filename":"YMJ27-1-031to041.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"YMJ27-1-031to041.pdf","objectType":"fulltext","url":"https://yamanashi.repo.nii.ac.jp/record/2795/files/YMJ27-1-031to041.pdf"},"version_id":"cc6d542f-4051-4072-b8a7-1d30309c39f3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sphingosine 1-phosphate","subitem_subject_language":"en"},{"subitem_subject":"Invasion","subitem_subject_language":"en"},{"subitem_subject":"Seprase","subitem_subject_language":"en"},{"subitem_subject":"Angiogenesis","subitem_subject_language":"en"},{"subitem_subject":"Extracellular matrix","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<Original article> Sphingosine 1-phosphate stimulates cell migration and active seprase expression in human endothelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<Original article> Sphingosine 1-phosphate stimulates cell migration and active seprase expression in human endothelial cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"5","path":["220"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-03-14"},"publish_date":"2013-03-14","publish_status":"0","recid":"2795","relation_version_is_last":true,"title":["<Original article> Sphingosine 1-phosphate stimulates cell migration and active seprase expression in human endothelial cells"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-10-11T07:20:58.499644+00:00"}