{"created":"2023-05-18T10:11:17.428395+00:00","id":3023,"links":{},"metadata":{"_buckets":{"deposit":"51e28f14-3dc5-442d-979e-e11d39b13dcc"},"_deposit":{"created_by":5,"id":"3023","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"3023"},"status":"published"},"_oai":{"id":"oai:yamanashi.repo.nii.ac.jp:00003023","sets":["205:251:267"]},"author_link":["13924","13925","13926","13927","13928","13929","13930","13931","13932","13933","13934","13935","13936","13937"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"16","bibliographicPageStart":"7","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"山梨医科大学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Yamanashi medical journal","bibliographic_titleLang":"en"}]}]},"item_10001_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"土田, 孝之","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金井, 直明","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"遠藤, 真一","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤, 梧郎","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"前田, 秀一郎","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上野, 精","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"橋本, 敬太郎","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"内容","attribute_value_mlt":[{"subitem_description":"プロスタグランジン(以下PG)製剤の大きな問題は,血液中に短時間しか存在できないことにある。PG製剤の多くは肺でトランスポーターという膜蛋白によって細胞内に取り込まれ不活性化される。この不活性化には15-hydroxy-PG-dehydrogenaseを中心に多くの細胞内代謝酵素が関与する。したがって半減期の長いPG製剤を開発する上で効率的な方法は,多くの代謝酵素それぞれに対して抵抗性を与えるよりも,細胞内に取り込まれない構造を持たせることである。我々はヒトPGトランスポーター(以下PGT)cDNAをHeLa細胞に導入した安定発現細胞を作成した。PGT安定発現細胞の3 H-PGE1(0.6nM)の細胞内取り込みは20分間でほぼプラトーに達した。この時点でPGT安定発現細胞はcontrol細胞に比べて,アルブミン非存在下で約150倍,5%アルブミン存在下で約80倍の3 H-PGE1を細胞内に取り込んだ。0.6nM PGE1のアルブミン結合率はほぼ100%である。それにもかかわらず,PGTがアルブミン存在下でもPGE1を取り込めることが明らかになり,生理的に大きな役割を果たしているものと推定された。この系を使いPGTを通過しない安定なPG製剤の構造を検討した。PG誘導体のPG受容体への親和性とPGTへの親和性を比較検討したが,その間に有意な相関は認められなかった。したがってPG受容体への親和性は高く維持しつつ,PGTへの親和性を低く抑えた半減期の長いPG製剤が開発可能であると考えられた。またPGTへの親和性を決定する重要な基はPG分子内の極性基であったため,その極性基の修飾が安定なPG製剤には必要であると思われた。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_full_name_24":{"attribute_name":"著者名(別表記)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"13931","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsuchida, Takayuki","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13932","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kanai, Naoaki","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13933","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Endou, Shinichi","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13934","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Katou, Gorou","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13935","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Maeda, Shuuichirou","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13936","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ueno, Akira","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"13937","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hashimoto, Keitarou","nameLang":"en"}]}]},"item_10001_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.34429/00003016","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"山梨医科大学医学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN10044054","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0912-0025","subitem_source_identifier_type":"ISSN"}]},"item_10001_text_4":{"attribute_name":"タイトルヨミ","attribute_value_mlt":[{"subitem_text_language":"ja-Kana","subitem_text_value":"ゲンチョ ヒト プロスタグランジントランスポーター HPGT ブンシ ノ セイシツ カラ エラレタ PG セイザイ ニ ユウヨウナ コウゾウ ノ ケントウ"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-27"}],"displaytype":"detail","filename":"AN10044054_14_01_02.pdf","filesize":[{"value":"110.3 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