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<Original> Effects of Sirtinol on Early Development of the Cloned Murine Embryos
https://doi.org/10.34429/00002834
https://doi.org/10.34429/0000283498d42557-0fbf-4623-abaa-31c214b8f510
名前 / ファイル | ライセンス | アクション |
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AA11839837_23_04_02.pdf (763.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-05-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | <Original> Effects of Sirtinol on Early Development of the Cloned Murine Embryos | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題 | SCNT (somatic cell nuclear transfer) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | cloned mouse | |||||
キーワード | ||||||
言語 | en | |||||
主題 | early development | |||||
キーワード | ||||||
言語 | en | |||||
主題 | HDACi (histone deacetylase inhibitor) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | sirtinol | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.34429/00002834 | |||||
ID登録タイプ | JaLC | |||||
著者名(別表記) | ||||||
姓名 | Hirata, Shuji(en) | |||||
著者名(別表記) | ||||||
姓名 | Fukasawa, Hiroko(en) | |||||
著者名(別表記) | ||||||
姓名 | Tagaya, Hikaru(en) | |||||
著者名(別表記) | ||||||
姓名 | Shoda, Tomoko(en) | |||||
著者名(別表記) | ||||||
姓名 | Wakayama, Teruhiko(en) | |||||
著者名(別表記) | ||||||
姓名 | Hoshi, Kazuhiko(en) | |||||
内容 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Since 1996, when Dolly was born, cloned vertebrates have been produced in many species. However, the success rate of animal cloning has remained very low especially in mice. This low rate is a severe hindrance in the application of animal cloning on various fields of studies. Recently, the treatment of reconstructed embryos with trichostatin A (TSA), one of the inhibitors for classes I and II histone deacetylases (HDACs), has been reported to significantly improve the development of cloned mice. In the present study, we have investigated the effect of sirtinol, an inhibitor for class III HDAC, on the early development of a cloned murine embryos. The embryos, reconstructed with an enucleated oocyte and cumulus cell nucleus, were treated with sirtinol during activation and/or for 12 hr after activation. The rate of blastocyst formation was significantly improved by treatment with 100 μM sirtinol for 12 hr after activation. Moreover, six live offspring were born after transfer of the cloned embryos, which were treated with TSA and sirtinol, into surrogate mothers. These resultsindicate that sirtinol improves the early development of cloned murine embryos without adverse effects on subsequent development. | |||||
言語 | ja | |||||
出版者 | ||||||
言語 | ja | |||||
出版者 | 山梨大学医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1348-5091 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11839837 | |||||
書誌情報 |
ja : 山梨医科学雑誌 en : Yamanashi medical journal 巻 23, 号 4, p. 97-107, 発行日 2008 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |