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<Original article> Sphingosine 1-phosphate stimulates cell migration and active seprase expression in human endothelial cells
https://doi.org/10.34429/00002788
https://doi.org/10.34429/000027880e8a7641-444e-41a9-af5b-dae2879577ef
名前 / ファイル | ライセンス | アクション |
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YMJ27-1-031to041.pdf (1.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-03-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | <Original article> Sphingosine 1-phosphate stimulates cell migration and active seprase expression in human endothelial cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Sphingosine 1-phosphate | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Invasion | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Seprase | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Angiogenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Extracellular matrix | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.34429/00002788 | |||||
ID登録タイプ | JaLC | |||||
著者名 |
Okamoto, Hirotaka
× Okamoto, Hirotaka× Yatomi, Yutaka× Satoh, Kaneo× Yamane, Tetsu× Mitsumata, Masako× Chen, Wen-Tien× Ozaki, Yukio× Fujii, Hideki |
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内容 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Objective: Sphingosine 1-phosphate (Sph-1-P), a bioactive lysophospholipid present in the plasma, is released from activated platelets. Our previous study demonstrated that Sph-1-P promoted the spreading on and migration of human umbilical vein endothelial cells (HUVEC) through the extracellular matrix (ECM), suggesting a possible induction of cell surface proteases in the Sph-1-P activated endothelial cells. In the present study, we examined whether seprase, a type II transmembrane serine protease (TTSP) usually absent in tissue cells, can be induced in endothelial cells activated by Sph-1-P. Methods: HUVEC migration through ECM was examined by modifi ed Boyden chamber assay. Western blotting and immunoprecipitation, using anti-seprase monoclonal antibodies (mAbs), were used to confi rm the seprase expression of HUVEC. Results: We show that Sph-1-P enhanced expression of active form seprase in a time- and dose-dependent manner in HUVEC. The Sph-1-P inducible active form seprase could be blocked by pertussis toxin and by C3 transferase, which inactivate Gi-type heterotrimetric G proteins and Rho, respectively. Conclusion: These results show that Sph-1-P can regulate migration of endothelial cells by inducing active form seprase expression, which, in turn, is mediated through a Gi-coupled cell surface receptor and the Rho protein. | |||||
言語 | en | |||||
出版者 | ||||||
言語 | ja | |||||
出版者 | 山梨大学医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1348-5091 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11839837 | |||||
書誌情報 |
ja : 山梨医科学雑誌 en : Yamanashi medical journal 巻 27, 号 1, p. 31-41, 発行日 2013 |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |